Τρίτη 18 Οκτωβρίου 2016

Reconstitution in vitro of the entire cycle of the mouse female germ lineΑνασύσταση ολόκληρου του αναπαραγωγικού κύκλου θηλυκών ποντικιών από βλαστικά κύτταρα γιά πρώτη φορά

Ανασύσταση ολόκληρου του αναπαραγωγικού κύκλου  θηλυκών  ποντικιών από βλαστικά κύτταρα, έξω από το σώμα του ζώου γιά πρώτη φορά στο εργαστήριο, από Ιάπωνες  γενετιστές επιστήμονες.
Η αναμενόμενη εφαρμογή (μετά από τα ερχόμενα δέκα περίπου χρόνια) αυτής της μεθόδου στον άνθρωπο εγείρει πολλά θέματα ηθικής, υγείας, ασφαλούς συνέχισης της ζωής  κι εξέλιξης του Ανθρώπου. μ.λ.π.

Nature | Letter
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Reconstitution in vitro of the entire cycle of the mouse female germ line

Naturedoi:10.1038/nature20104
ReceivedAccepted Published online
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The female germ line undergoes a unique sequence of differentiation processes that confers totipotency to the egg1, 2. The reconstitution of these events in vitro using pluripotent stem cells is a key achievement in reproductive biology and regenerative medicine. Here we report successful reconstitution in vitro of the entire process of oogenesis from mouse pluripotent stem cells. Fully potent mature oocytes were generated in culture from embryonic stem cells and from induced pluripotent stem cells derived from both embryonic fibroblasts and adult tail tip fibroblasts. Moreover, pluripotent stem cell lines were re-derived from the eggs that were generated in vitro, thereby reconstituting the full female germline cycle in a dish. This culture system will provide a platform for elucidating the molecular mechanisms underlying totipotency and the production of oocytes of other mammalian species in culture.

At a glance Figures

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  1. Oocyte production from pluripotent stem cells.
    Figure 1
  2. Transcriptome analysis of oogenesis in vitro.
    Figure 2
  3. Offspring from in-vitro-generated eggs. Figure 3
  4. Reconstitution of the second cycle of oogenesis in vitro.
    Figure 4
  5. Evaluation of the basal culture conditions.
    Extended Data Fig. 1
  6. Meiotic progression and establishment of genomic imprints in culture.
    Extended Data Fig. 2
  7. Isolation of individual 2FLs for IVG culture and MII oocytes from IVM culture.
    Extended Data Fig. 3
  8. Expression of a specific class of LTR.
    Extended Data Fig. 4
  9. Growth and fertility of offspring from in-vitro-generated oocytes.
    Extended Data Fig. 5
  10. Developmental potential of in-vitro-generated oocytes.
    Extended Data Fig. 6
  11. Oogenesis in vitro using other ESC and iPSC lines.
    Extended Data Fig. 7
  12. Summary of eye colour and transgene of the pups derived from in-vitro-generated oocytes.
    Extended Data Fig. 8
  13. Chimaera analysis of rESCs.
    Extended Data Fig. 9
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